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ORIGINAL ARTICLE
Year : 2014  |  Volume : 30  |  Issue : 2  |  Page : 126-131

Morphological and electron microscopic effects of mitomycin C inhalation in experimentally induced laryngeal fibrosis in dogs


1 Otolaryngology - Head and Neck Surgery Department, Suez Canal University, Ismailia, Egypt
2 Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
3 Pathology Department, Suez Canal University, Ismailia, Egypt
4 Histology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Correspondence Address:
Mohamed T El Tabbakh
Otolaryngology - Head and Neck Surgery Department, Faculty of Medicine, Suez Canal University, 41522
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1012-5574.133214

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Purpose This study was carried out to investigate the morphological and ultrastructural effects of mitomycin C in the inhalational form on induced laryngeal tissue injuries and fibrosis. Materials and methods This study was carried out on 17 cross-breed clinically healthy dogs. Three dogs were included in a normal control group. The rest of the 14 dogs were subjected to induction of laryngeal fibrosis and assigned randomly to two equal groups: one treated with inhalational mitomycin C and the other was an untreated control group. Tissue biopsies were studied after H&E and Masson's trichrome stains, whereas other pieces were examined by electron microscopy. Results Epithelial ulceration with marked inflammatory infiltration, edema, and congestion was evident in all experimented dogs. There was a significant difference in epithelial hyperplasia between the treated and the untreated groups; it was markedly reduced in the treated group. The untreated group showed extensive well-organized fibrosis that was significantly increased compared with the other groups. Results were confirmed by electron microscopy. Conclusion Inhalation of mitomycin C has a potent antifibrotic effect through decreasing the activity of fibroblast evident by the decrease in both the size of fibroblast and the density of the intrafibroblast endoplasmic reticulum.


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